1. EZ-Vision DNA Dye as Loading Buffer EZ-Vision DNA染液 + 6x上样缓冲液(混合在一个管里) 无需配胶时加染料 只需上样、跑胶,电泳后即可在紫外下观察DNA条带 用量:6X , 即1ul EZ-Vision + 5ul DNA样品 提供三种上样缓冲液,分别含1、2、3条电泳指示带 |
品名及描述 | 级别 | 货号 | 包装 | 价格(元) | EZ Vision One DNA Dye as Loading Buffer EZ Vision DNA染料上样缓冲液 (含1条指示带) 指示带近似位置为:10bp | 生物技术级 生物技术级 生物技术级 | amresco N472-Q amresco N472 amresco N472 | 0.5ml 1ml 5x 1ml | 408 450 1806 | EZ Vision Two DNA Dye as Loading Buffer EZ Vision DNA染料上样缓冲液 (含2条指示带) 指示带近似位置为:400bp, 4000 | 生物技术级 | amresco N650 | 5x 1ml | 1625 | EZ Vision Three DNA Dye as Loading Buffer EZ Vision DNA染料上样缓冲液 (含3条指示带) 指示带近似位置为:10bp, 400bp, 4000bp | 生物技术级 生物技术级 | amresco N313 amresco N313 | 1ml 5x 1ml | 385 1701 | EZ Vision Sample Kit EZ Vision DNA套装 含N472-1ml, N313-1ml | 生物技术级 | amresco N473-2管 | 1套 | 755 | EZ Vision Sample Kit EZ Vision DNA套装 含N472-1ml, N650-1ml, N473-1ml | 生物技术级 | amresco N473-3管 | 1套 | 1026 |
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2. EZ-Vision In-Gel Solution, 10,000 x EZ-Vision 染液, 10,000 x (加在胶内,与EB用法一样) 与EB 用法一样,溶胶时加在胶内,稀释比例为1:10000。 |
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品名及描述 | 级别 | 货号 | 包装 | 价格(元) | EZ Vision In-Gel Solution, 10000 x EZ Vision 染液, 10000 x | 生物技术级 | amresco N391-0.5ml | 0.5ml | 1132 |
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EZ-Vision, DNA Dye as Loading Buffer, 6X EZ-Vision is a non-mutagenic fluorescent reagent that produces instant visualization of DNA bands upon UV illumination of agarose gels. EZ-Vision is non-mutagenic and non-toxic with no hazardous shipping, handling or disposal costs. Supplied in AMRESCO’s 6X DNA Loading Buffer, EZ-Vision forms a tight complex with the sample DNA and co-migrates with it during electrophoresis. Post-run staining and destaining is completely eliminated and results can be visualized immediately after the run by placing the gel on a standard UV transilluminator. It is ideal for applications needing rapid DNA band visualization and for environments requiring a safe, non-hazardous alternative to Ethidium Bromide. EZ-Vision is available in 3 versions that differ only by the tracking dyes included in the loading buffer. EZ-Vision Three contains 3 tracking dyes that migrate at 4,000 bp, 400 bp, and 10 bp. EZ-Vision Two contains 2 tracking dyes that migrate at 4,000 bp and 400 bp. EZ-Vision One contains only a single fast-running tracking dye that migrates at approximately 10 bp in a 1% agarose gel. |
Available with 3 different tracking dye options! | EZ-Vision One, Two and Three. Left image: 1% TAE agarose gel showing the fluorescence of AMRESCO’s 1kb Ladder (K180-250μl) stained with EZVision One (lane 1), EZ-Vision Two (lane 2) and EZ-Vision Three (lane3) captured with Syngene GBox-HR Gel Doc System using SP filter selection. Right image: Digital camera photograph of the same gel as left image, showing the colors and migration position of the one fast migrating tracking dye of EZ-Vision One (lane 1), the two tracking dyes of EZ-Vision Two (lane2), and the three tracking dyes of EZ-Vision Three (lane 3). |
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EZ-Vision sensitivity compared to Ethidium Bromide | DNA MW Marker, 1KB Ladder was mixed with EZ-Vision Two, DNA Dye as Loading Buffer, 6X or a loading buffer containing 0.5 μg/mL ethidium bromide. After electrophoresis, the gel was visualized on a standard UV illuminator. |
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EZ-Vision Environmental Toxicity Testing | EZ-Vision environmental hazard testing was determined by the CCR Title 22 Fathead Minnow Hazardous Waste Screen Bioassay. Both EZ-Vision Two and EZ-Vision? Three were determined non-hazardous with LC50 > 750 mg/l. |
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Relative migration of prestained samples of dsDNA in 1% agarose gel |
| The data shows that EZ-Vision has much less effect on DNA migration than Ethidium Bromide or Competitor 2 Green Dye. Samples stained with EZ-Vision are not impeded and migrate similar to dsDNA that is stained post electrophoresis |
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